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Figure 5: (a–g) A photomicrograph of fluoxetine and omega-3-treated testis showing (a) histological features nearly similar to the control group, but some germ cells have vacuolated cytoplasm (arrow). The intertubular space contains acidophilic material (A) (H and E ×400); (b) several layers of the spermatogenic cells at different stages; spermatogonia (SP), primary spermatocytes (1ry), spermatids with vacuolated cytoplasm (D), Sertoli cells (R) and spermatozoa (S) appear in the lumen (H and E ×1000); (c) moderate collagen fiber deposition in the capsule (arrow), interstitial tissues (I), and in basal lamina (*) (MT ×200); (d) moderate PAS reaction in basal amina (arrow) with PAS-positive materials deposition in-between tubules (*) (PAS ×200); (e) weak caspase-3 immunostaining in some germ cells (arrow) (caspase-3 ×400); (f) many positive proliferating cell nuclear antigen (PCNA) immunostaining nuclei of the germ cells (PCNA ×400); and (g) intense androgen receptor (AR) immunoexpression in the interstitial cells (I) and myoid cells (M) but weak in sertoli cells (R) (AR ×400). MT, Masson Trichome; PAS, Periodic Acid Schiff's reaction. |
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