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  Indian J Med Microbiol
 

Figure 4: (a–g) A photomicrograph of recovery testis showing (a) degenerated seminiferous tubules with few germ cells (arrow) and containing no sperm. However, the central tubule (T) contains many spermatogenic cells. Intertubular space contains homogeneous acidophilic material (A) and vacuoles (V) (H and E ×200). (b) Rounded seminiferous tubule bounded by semiregular basal lamina and lined by many spermatogenic cells, but some showed pyknotic nuclei (PK). The intertubular space contains acidophilic material (A) and vacuoles (V) (H and E ×400); (c) marked collagen fibers deposition in the capsule (arrow), around blood vessels (BV), interstitial tissues (I), and in the basal lamina (*) (MT ×200); (d) strong PAS reaction in basal lamina (black arrow) and around blood vessels (white arrow) (PAS ×200); (e) some cells with intense positive cytoplasmic caspase-3 immunostaining (white arrows), whereas others show weak immunostaining (black arrow) (caspase-3 ×400); (f) some positive proliferating cell nuclear antigen (PCNA) immunostained germ cells (PCNA ×400); (g) weak androgen receptor (AR) nuclear immunoexpression in the interstitial cells (I) and myoid cell (M) (AR ×400). MT, Masson Trichome; PAS, Periodic Acid Schiff's reaction.

Figure 4: (a–g) A photomicrograph of recovery testis showing (a) degenerated seminiferous tubules with few germ cells (arrow) and containing no sperm. However, the central tubule (T) contains many spermatogenic cells. Intertubular space contains homogeneous acidophilic material (A) and vacuoles (V) (H and E ×200). (b) Rounded seminiferous tubule bounded by semiregular basal lamina and lined by many spermatogenic cells, but some showed pyknotic nuclei (PK). The intertubular space contains acidophilic material (A) and vacuoles (V) (H and E ×400); (c) marked collagen fibers deposition in the capsule (arrow), around blood vessels (BV), interstitial tissues (I), and in the basal lamina (*) (MT ×200); (d) strong PAS reaction in basal lamina (black arrow) and around blood vessels (white arrow) (PAS ×200); (e) some cells with intense positive cytoplasmic caspase-3 immunostaining (white arrows), whereas others show weak immunostaining (black arrow) (caspase-3 ×400); (f) some positive proliferating cell nuclear antigen (PCNA) immunostained germ cells (PCNA ×400); (g) weak androgen receptor (AR) nuclear immunoexpression in the interstitial cells (I) and myoid cell (M) (AR ×400). MT, Masson Trichome; PAS, Periodic Acid Schiff's reaction.