Figure 3: (a–j) A photomicrograph of fluoxetine-treated testis showing (a) disorganized germ cell layers with large cytoplasmic vacuoles (arrow), few spermatozoa inside the tubules (S), and homogeneous acidophilic material (A) in-between the tubules (H and E ×200), and (b) disorganized germ cells with deeply stained pyknotic nuclei (PK) and cytoplasmic vacuolation (V). The lumen of tubules contains no spermatozoa (L). Leydig cells (I) show pyknotic nuclei (H and E ×400); (c) degenerated seminiferous tubules (arrow) and degenerated interstitial tissue (I). Notice congested blood vessel (BV) in-between the tubules (H and E ×200). (d) Distorted seminiferous tubules surrounded by irregular notched basal lamina (arrow). Distorted Leydig cells (I) and congested blood vessels (BV) in the wide intertubular space (H and E ×400). (e) Degenerated spermatogenic cells (arrow) and some cells are replaced by vacuoles (V). The tubule contains acidophilic hyaline streaks (A) extending between the degenerated spermatogenic cells (H and E ×400). (f) Marked increase of the collagen fibers in the capsule (arrow), the interstitial tissues (I), and in the basal lamina (*) (MT ×200); (g) very strong PAS reaction in the basal lammina (black arrow) and around blood vessels (white arrow) with strong PAS-positive materials in-between the tubules (*) (PAS ×200); (h) intense positive caspase-3 cytoplasmic immunostaining (arrow) (caspase-3 ×400); (i) few positive proliferating cell nuclear antigen (PCNA) immunostaining cells (PCNA ×400); (j) negative immunoexpression of androgen receptor (AR ×400). MT, Masson Trichome; PAS, Periodic Acid Schiff's reaction.