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Figure 2: (a) A TEM photo of the macroscopic Sarcocystis fusiformis cyst wall revealed villar protrusions (VP), electron-dense granules (g) within the ground substance (GS) and cyst merozoites (CM). (b) A TEM photo of the macroscopic S. fusiformis cyst from the esophageal wall of a buffalo showed dense granules forming the cyst covering and septal meshwork (M). The large elongated merozoites had a nucleus (N) in the posterior portion and numerous polysaccharide granules (P) with a diameter of 0.2 mm in the middle portion. The anterior part of the merozoites was rich in micronemes (m) and rhobtries (r). A conoid (C) structure was present at the anterior end. The developing merozoites were seen with the internal structures attaching directly to the septal meshwork (black arrow). (c) An infected rat's stool (1 week postinfection) stained by iodine showed oval-shaped sporocysts (11×7 mm) (SPC) (group IV) (×1000). (d) Wet mounts of a rat's stool (2 weeks postinfection) showed an oocyst (16×12.5 mm) with the oocyst wall (O) and contained two sporocysts (SPC) (g roup V) (×1000). (e) An infected rat's stool (1 month postinfection) stained by Geimsa stain showed the oocyst with the oocyst wall (OW), containing two sporocysts (SPC) (group VI) ( × 1000). TEM, transmission electron microscope. |
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