Figure 2: Agarose gel electrophoresis of PCR product after amplification and digestion by restriction enzyme (Eco RI). Lane 1, the DNA marker 100 bp (ladder); lane 2, negative control (no DNA was added); lane 4, positive heterozygous polymorphism with one band at 112 bp (E⁺/E^- alleles); lane 6, positive heterozygous polymorphism with one band at 510 bp (E^-/E⁺ alleles); lane 7, homozygous negative polymorphism (E^-/E^- alleles) (no bands neither at 112 bp nor at 510 bp, with an uncut DNA at 600 bp); lane 3, lane 5, and lane 8, positive homozygous polymorphism (E⁺/E⁺ alleles) with two bands at 112 and 510 bp