Year : 2016 | Volume
: 29 | Issue : 4 | Page : 826--834
Profiling of microRNA-122 in chronic hepatitis C
Enas M Ghoneim1, Aza M Abed El-Aziz1, Tawfik M Abd El-Mottaleb1, Ghada R El-Hendawy2, Hossam M El-Ezawy3, Fatma O Khalil1
1 Department of Medical Microbiology and Immunology, National Liver Institute, Menoufia, Egypt
2 Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Menoufia, Egypt
3 Department of Biochemistry, National Liver Institute, Menoufia, Egypt
The aim of this study was to assess the expression level of microRNA-122 (miRNA-122) in the serum as a diagnostic marker of liver injury in chronic hepatitis C, liver cirrhosis, and hepatocellular carcinoma (HCC).
MicroRNAs are small RNA molecules including the liver specific, miRNA-122, which acts as a critical regulator of hepatic gene expression and an essential factor for hepatitis C virus (HCV) replication.
Patients and methods
A total of 112 participants who were divided into four groups were included in the study. Group I included 27 patients with chronic hepatitis C infection. Group II included 27 patients with HCC. Group III included 30 patients with chronic liver cirrhosis. Group IV included 18 healthy individuals without hepatitis C infection or any other disease who served as a control group. Liver function tests, α-fetoprotein, complete blood count, hepatitis C antibody, hepatitis B surface antigen, HCV-RNA using PCR, and detection of miRNA-122 in the serum using real-time PCR were carried out for all groups.
The expression level of serum miRNA-122 displayed a significant fold increase in serum miRNA-122 in the HCV group compared with the other groups (P ≤ 0.0001). Patients with HCC showed a nonsignificantly higher expression level of miRNA-122 as compared with controls. miRNA-122 fold change at cutoff less than 1.64 could predict patients with HCC with 81.4% sensitivity and 48% specificity.
Measurements of serum miRNA-122 may be useful in the evaluation of HCV patients, by permitting with a serum test, the diagnosis of HCC with high predictivity and sensitivity.
Fatma O Khalil
Department of Medical Microbiology and Immunology, National Liver Institute, Menoufia University, Kafr Nafra Village, Berket El Sabae, Menoufia
|How to cite this article:|
Ghoneim EM, Abed El-Aziz AM, Abd El-Mottaleb TM, El-Hendawy GR, El-Ezawy HM, Khalil FO. Profiling of microRNA-122 in chronic hepatitis C.Menoufia Med J 2016;29:826-834
|How to cite this URL:|
Ghoneim EM, Abed El-Aziz AM, Abd El-Mottaleb TM, El-Hendawy GR, El-Ezawy HM, Khalil FO. Profiling of microRNA-122 in chronic hepatitis C. Menoufia Med J [serial online] 2016 [cited 2022 Oct 3 ];29:826-834
Available from: http://www.mmj.eg.net/article.asp?issn=1110-2098;year=2016;volume=29;issue=4;spage=826;epage=834;aulast=Ghoneim;type=0