Menoufia Medical Journal

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 29  |  Issue : 4  |  Page : 826--834

Profiling of microRNA-122 in chronic hepatitis C


Enas M Ghoneim1, Aza M Abed El-Aziz1, Tawfik M Abd El-Mottaleb1, Ghada R El-Hendawy2, Hossam M El-Ezawy3, Fatma O Khalil1 
1 Department of Medical Microbiology and Immunology, National Liver Institute, Menoufia, Egypt
2 Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Menoufia, Egypt
3 Department of Biochemistry, National Liver Institute, Menoufia, Egypt

Correspondence Address:
Fatma O Khalil
Department of Medical Microbiology and Immunology, National Liver Institute, Menoufia University, Kafr Nafra Village, Berket El Sabae, Menoufia
Egypt

Objective The aim of this study was to assess the expression level of microRNA-122 (miRNA-122) in the serum as a diagnostic marker of liver injury in chronic hepatitis C, liver cirrhosis, and hepatocellular carcinoma (HCC). Background MicroRNAs are small RNA molecules including the liver specific, miRNA-122, which acts as a critical regulator of hepatic gene expression and an essential factor for hepatitis C virus (HCV) replication. Patients and methods A total of 112 participants who were divided into four groups were included in the study. Group I included 27 patients with chronic hepatitis C infection. Group II included 27 patients with HCC. Group III included 30 patients with chronic liver cirrhosis. Group IV included 18 healthy individuals without hepatitis C infection or any other disease who served as a control group. Liver function tests, α-fetoprotein, complete blood count, hepatitis C antibody, hepatitis B surface antigen, HCV-RNA using PCR, and detection of miRNA-122 in the serum using real-time PCR were carried out for all groups. Results The expression level of serum miRNA-122 displayed a significant fold increase in serum miRNA-122 in the HCV group compared with the other groups (P ≤ 0.0001). Patients with HCC showed a nonsignificantly higher expression level of miRNA-122 as compared with controls. miRNA-122 fold change at cutoff less than 1.64 could predict patients with HCC with 81.4% sensitivity and 48% specificity. Conclusion Measurements of serum miRNA-122 may be useful in the evaluation of HCV patients, by permitting with a serum test, the diagnosis of HCC with high predictivity and sensitivity.


How to cite this article:
Ghoneim EM, Abed El-Aziz AM, Abd El-Mottaleb TM, El-Hendawy GR, El-Ezawy HM, Khalil FO. Profiling of microRNA-122 in chronic hepatitis C.Menoufia Med J 2016;29:826-834


How to cite this URL:
Ghoneim EM, Abed El-Aziz AM, Abd El-Mottaleb TM, El-Hendawy GR, El-Ezawy HM, Khalil FO. Profiling of microRNA-122 in chronic hepatitis C. Menoufia Med J [serial online] 2016 [cited 2022 Oct 3 ];29:826-834
Available from: http://www.mmj.eg.net/article.asp?issn=1110-2098;year=2016;volume=29;issue=4;spage=826;epage=834;aulast=Ghoneim;type=0