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Year : 2014  |  Volume : 27  |  Issue : 1  |  Page : 50-54

Study of DNA damage in asphyxiated newborns

Department of Pediatrics, Faculty of Medicine, Menofiya University, Egypt

Correspondence Address:
Hany M. El-Boshy
Msc, Samanoud, 36793, Gharbia
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1110-2098.132739

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Objective The present study was carried out to assess the presence of DNA damage in asphyxiated newborns. Background Perinatal asphyxia is one of the major causes of neonatal mortality. It results from lack of oxygen before, during, or after birth. Patients and methods Thirty asphyxiated and 20 nonasphyxiated neonates were included in this case-control study. Blood samples were collected from a peripheral vein into heparinized tubes and stored at 10°C away from light to prevent DNA damage and were processed within 24 h to avoid further DNA damage. Estimation of DNA damage in peripheral leukocytes by DNA fragmentation assay was carried out by DNA extraction and then gel electrophoresis. Results Significant DNA damage was found (56.7%) in asphyxiated newborns and positively correlated with severity and clinical complications of hypoxic-ischemic encephalopathy. Conclusion The study indicates the presence of DNA damage in asphyxiated newborns.

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