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Figure 1: (a) A photomicrograph of image J analyzer showing the counting of positive proliferating cell nuclear antigen (PCNA) nuclei (PCNA ×200). (b–f) A photomicrograph of control testis showing (b) regularly arranged seminiferous tubules containing different germ cells and mature spermatozoa (S). Notice the connective tissue capsule (arrow) (H and E ×200). (c) Seminiferous tubule containing spermatogonia (SP), primary spermatocytes (1ry), spermatides (D), Sertoli cells (R), and spermatozoa (S). The myoid cells (M) are outside the basal lamina (H and E ×1000). (d) Seminiferous tubules with regular basal lamina (arrow) containing mature spermatozoa (S). Notice: clusters of Leydig cells with acidophilic cytoplasm in-between (I). (H and E ×400). (e) Minimal collagen fibers in the testicular capsule (arrow), interstitial tissues (I), and basal lamina (*) (MT ×200). (f) Moderate PAS reaction in the basement membrane of seminiferous tubules (arrow) (PAS ×200). MT, Masson Trichome; PAS, Periodic Acid Schiff's reaction. |
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