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Figure 5: Photomicrographs of sections of testis of the tramadol and vitamin C-treated group showing that most of seminiferous tubules appear normal (arrow) while few tubules are affected (arrowhead) (a; H and E, ×100). More or less normal seminiferous tubules with normal spermatogenic cells (arrow) and a lumen full of sperms (asterisk) (b; H and E, ×400). Mild to moderate positive PAS reaction in the basement membrane (arrow), spermatogenic cells (sp), sperms (sper), and interstitium (tailed arrow) is noticed (c; PAS, ×200). Some collagen fibers (blue color) around the blood vessel (arrow) and mild thickening of tunica albuginea (arrowhead) can be noticed (d; Masson's trichrome, ×200). H and E, hematoxylin and eosin. |
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