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Figure 5: Immunohistochemical staining of cardiac myoglobin in the different studied groups: (a) Sections from the control group showing positive myoglobin expression in cardiac muscle, where the brown pigment strongly appears in both the nucleus and the cytoplasm of the cardiac muscles, indicating that the viable cells retained myoglobin. (b) Sections from the isoprenaline (ISO)-treated group showed negative myoglobin expression in cardiac muscles; these tissues did not stain, indicating that they contained no myoglobin. (c) Sections from rats pretreated with quercetin showed mild myoglobin expression in cardiac muscle; the brown pigment mildly appeared in both the nucleus and the cytoplasm of the cardiac muscles. (d) Sections from rats pretreated with trimetazidine showed moderate myoglobin expression; the brown pigment moderately appeared in both the nucleus and the cytoplasm of the cardiac muscles. (e) Sections from rats that received both drugs showed strong myoglobin expression; the brown pigment strongly appeared in both the nucleus and the cytoplasm of the cardiac muscles. |
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