ORIGINAL ARTICLE |
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Year : 2017 | Volume
: 30
| Issue : 3 | Page : 940-945 |
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Interleukin 10 gene polymorphism and lepra reactions: susceptibility and immunological correlation
Alaa H Maraee1, Azza G Antar1, Rania M. A. El-Shazly2, Shaimaa F. G. El-Sayed MSc 3
1 Department of Dermatology, Andrology and STIs, Menoufia University, Menoufia, Egypt 2 Department of Biochemistry, Faculty of Medicine, Menoufia University, Menoufia, Egypt 3 Department of Dermatology, Albagoor General Hospital, Menoufia, Egypt
Correspondence Address:
Shaimaa F. G. El-Sayed Department of Dermatology, Albagoor General Hospital, Albagoor, Menoufia, 32821 Egypt
Source of Support: None, Conflict of Interest: None | Check |
DOI: 10.4103/1110-2098.218295
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Objectives
The aim of the study was to evaluate interleukin 10 (IL-10) gene single nucleotide polymorphisms (SNPs) as a susceptibility gene in lepra reactions.
Background
Leprosy is a chronic dermatoneurological disease, caused by Mycobacterium leprae. Lepra reactions are immune inflammatory-related leprosy phenotypes that may occur before diagnosis of leprosy, during treatment, or after multiple drug therapy. IL-10 gene becomes widely used in studies of immune-mediated disease. It can be a marker of disease susceptibility as well as of disease severity. This work studies IL-10 gene polymorphisms as a susceptibility gene in lepra reactions which may be useful in preventing lepra reactions as a complication of leprosy.
Patients and methods
This study was conducted on 40 patients with leprosy (20 with reactions), who were recruited from the Menoufia Unit of Dermatology and Leprosy within a period of 1 year, in addition to 20 control subjects, without history of leprosy, from the same geographic area. SNPs –1082A/G, –819C/T, and –592 A/C were identified in cases and control groups by PCR-RFLP.
Results
The genotype C/C in the SNP-819 was associated with lepra reaction (P < 0.01). There was no association between –592 A/C (P > 0.05) and 1082A/G (P > 0.05) and lepra reaction. The haplotype –1082A–819C–592C/–1082A–819T-592A is considered a risky haplotype category. The study documents that –1082A–819C–592C/–1082A–819C–592C and –1082A–819T–592A/–1082A–819T–592A are considered protective haplotypes. According to the study, the haplotype – 1082G–819C–592C/–1082A–819C–592C has no effect genetically.
Conclusion
IL-10 gene 819 SNP (C/C) is a risky polymorphism which increases susceptibility to lepra reaction, whereas IL-10 1082 SNP and IL-10 592 SNP are not associated with lepra reactions. |
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